Part:BBa_K5242013
Cre
Cre recombinase, a site-specific recombinase enzyme, plays a crucial role in genetic engineering and biological research. It is derived from the P1 bacteriophage and is known for its ability to catalyze the recombination of DNA sequences known as loxP sites. This enzyme functions by creating a double-strand break at the loxP sites, followed by the re-ligation of the DNA strands, resulting in the exchange, insertion, or deletion of genetic material.
In our design, we incorporated the Cre recombinase gene downstream of the sensor and designed loxP sequences on either side of the suicide gene. Under specific conditions like heat stress, the concentration of stress response gene transcipts is high, which can form duplex with sensor. thus, ADAR is able to edits the stop codon of the ogRNA sequence in the sensor, allowing for the expression of Cre recombinase. The Cre recombinase can knockout the suicide gene, preventing the death of the strain. When conditions change, Cre recombinase is not expressed in large quantities, so the suicide gene is continuously expressed and accumulates, eventually leading to the death of the strain. This method avoids the drawbacks of persistent leakage expression of the suicide gene, which could otherwise damage the cells.Figure.1 shows the structure of our Strain Security System.
Figure 1.The design of Strain Security System
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 356
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 42
- 1000COMPATIBLE WITH RFC[1000]
| None |